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Laser Microdissection

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Laser Microdissection system

Laser Micro Dissection systems combine a microscope for observation and a laser for dissection.  The goal of the system is to prepare pure and homogeneous sample from tissues, biopsies or cultured cells. After laser dissection, these small samples can be used for genomic, transcriptomic and proteomic analysis.

The microscope utilizes a motorized stage with xyz motion capabilities mounted with a (optional) fluorescence light. Slides are mounted inverted onto the microscope stage, where the desired cellular components are excised from the slide by the UV laser passing through the glass to cut the foil membrane. Gravity facilitates the capture of the dissected cellular components directly into tubes located in a substage tube holder. The UMCG currently has an LMD system from Leica (LMD6500).

 

  


 Video demonstrating the Leica system

Information:
Please contact Wierd Kooistra (Contact, 050-3619639) for more information on the LDM system, instructions or technical support for setting up a small pilot study.

Articles published by different groups of the UMCG using Laser microdissection technique. 


(q)RT-PCR
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A chronic obstructive pulmonary disease related signature in squamous cell lung cancer.
Boelens MC, Gustafson AM, Postma DS, Kok K, van der Vries G, van der Vlies P, Spira A, Lenburg ME, Geerlings M, Sietsma H, Timens W, van den Berg A, Groen HJ.
Lung Cancer. 2011 May;72(2):177-83. Epub 2010 Sep 15

RhoJ is an endothelial cell-restricted Rho GTPase that mediates vascular morphogenesis and is regulated by the transcription factor ERG
Yuan L, Sacharidou A, Stratman AN, Le Bras A, Zwiers PJ, Spokes K, Bhasin M, Shih SC, Nagy JA, Molema G, Aird WC, Davis GE, Oettgen P
.
Blood. 2011 Jul 28;118(4):1145-53. Epub 2011 May 31.
 

Vascular endothelial growth factor receptor 2 (VEGFR-2) signalling activity in paediatric pilocytic astrocytoma is restricted to tumour endothelial cells.
Sikkema AH, de Bont ES, Molema G, Dimberg A, Zwiers PJ, Diks SH, Hoving EW, Kamps WA, Peppelenbosch MP, den Dunnen WF.
Neuropathol Appl Neurobiol. 2011 Aug;37(5):538-48. doi: 10.1111/j.1365-2990.2011.01160.x.

Current smoking-specific gene expression signature in normal bronchial epithelium is enhanced in squamous cell lung cancer.
Boelens MC, van den Berg A, Fehrmann RS, Geerlings M, de Jong WK, te Meerman GJ, Sietsma H, Timens W, Postma DS, Groen HJ.
J Pathol. 2009 Jun;218(2):182-91.

Revertant Mosaicism Due to a Second-Site Mutation in COL7A1 in a Patient with Recessive Dystrophic Epidermolysis Bullosa. Stem cell-related cardiac gene expression early after murine myocardial Infarction.
Vandervelde S, van Luyn MJ, Rozenbaum MH, Petersen AH, Tio RA, Harmsen MC.
Cardiovasc Res. 2007 Mar 1;73(4):783-93. Epub 2006 Nov 29. 

Cigarette smoke-induced emphysema: A role for the B cell?
Am J Respir Crit Care Med.
2006 Apr 1;173(7):751-8. Epub  2006 Jan
van der Strate BW, Postma DS, Brandsma CA, Melgert BN, Luinge MA, Geerlings M, Hylkema MN, van den Berg A, Timens W, Kerstjens HA.
Am J Respir Crit Care Med. 2006 Apr 1;173(7):751-8. Epub 2006 Jan 6. 



Microarrays
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Modeling inducible human tissue neoplasia identifies an extracellular matrix interaction network involved in cancer progression.
Reuter JA, Ortiz-Urda S, Kretz M, Garcia J, Scholl FA, Pasmooij AM, Cassarino D, Chang HY, Khavari PA
.
Cancer Cell. 2009 Jun 2;15(6):477-88.
 

Expression of EpCAM is up-regulated during regeneration of renal
epithelia.
Trzpis M, McLaughlin PM, van Goor H, Brinker MG, van Dam GM, de Leij LM, Popa ER, Harmsen MC.
J Pathol. 2008 Oct;216(2):201-8. 


DNA
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Revertant mosaicism due to a second-site mutation in COL7A1 in a patient with recessive dystrophic epidermolysis bullosa.
Pasmooij AM, Garcia M, Escamez MJ, Nijenhuis AM, Azon A, Cuadrado-Corrales N, Jonkman MF, Del Rio M.
J Invest Dermatol. 2010 Oct;130(10):2407-11. Epub 2010 Jun 24.

Multiple correcting COL17A1 mutations in patients with revertant mosaicism of epidermolysis bullosa.
Pasmooij AM, Pas HH, Deviaene FC, Nijenhuis M, Jonkman MF.
Am J Hum Genet. 2005 Nov;77(5):727-40. Epub 2005 Sep 9. 

TP53 gene mutations in Hodgkin lymphoma are infrequent and not associated with absence of Epstein-Barr virus.
Maggio EM, Stekelenburg E, Van den Berg A, Poppema S.
Int J Cancer. 2001 Oct 1;94(1):60-6. 

Genomic aberrations in squamous cell lung carcinoma related to lymph node or distant metastasis.
Boelens MC, Kok K, van der Vlies P, van der Vries G, Sietsma H, Timens W, Postma DS, Groen HJ, van den Berg A.
Lung Cancer. 2009 Dec;66(3):372-8. Epub 2009 Mar 25.

More info on the LMD system can be found at the Leica website:
http://www.leica-microsystems.com/products/light-microscopes/life-science-research/laser-microdissection/details/product/leica-lmd7000/publications/

 

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